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specified nucleofection buffer  (Amaxa)

 
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    Amaxa specified nucleofection buffer
    Phenotypic analysis of DCs. At 24 h after <t>nucleofection,</t> DCs were harvested and stained with mAbs against DCs surface markers or appropriate isotype control antibody as described in “Materials and methods”. Percentage of positive DCs is indicated. One representative experiment of five is shown
    Specified Nucleofection Buffer, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/specified nucleofection buffer/product/Amaxa
    Average 90 stars, based on 1 article reviews
    specified nucleofection buffer - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Enhanced activation of human dendritic cells by silencing SOCS1 and activating TLRs simultaneously"

    Article Title: Enhanced activation of human dendritic cells by silencing SOCS1 and activating TLRs simultaneously

    Journal: Cancer Immunology, Immunotherapy : CII

    doi: 10.1007/s00262-012-1218-4

    Phenotypic analysis of DCs. At 24 h after nucleofection, DCs were harvested and stained with mAbs against DCs surface markers or appropriate isotype control antibody as described in “Materials and methods”. Percentage of positive DCs is indicated. One representative experiment of five is shown
    Figure Legend Snippet: Phenotypic analysis of DCs. At 24 h after nucleofection, DCs were harvested and stained with mAbs against DCs surface markers or appropriate isotype control antibody as described in “Materials and methods”. Percentage of positive DCs is indicated. One representative experiment of five is shown

    Techniques Used: Staining, Control

    Th1 cytokines secreted by pshS1NH–DCs were higher than those secreted by non-nucleofected DCs and pNC-GFP-DCs. 1 non-nucleofected DCs; 2 pNC-GFP-DCs; 3 pshS1NH–DCs. At 48 h after nucleofection, supernatants were collected for TNF-α, IL-6, IL-1β and IL-12p70 ELISA analyses. The pshS1NH–DCs secreted significantly higher level of Th1 cytokines than the pNC-GFP-DCs and non-nucleofected DCs (P < 0.05). Data shown are the means ± SEM and representative of six independent experiments. The significant differences between pshS1NH–DCs and control were determined by ANOVA
    Figure Legend Snippet: Th1 cytokines secreted by pshS1NH–DCs were higher than those secreted by non-nucleofected DCs and pNC-GFP-DCs. 1 non-nucleofected DCs; 2 pNC-GFP-DCs; 3 pshS1NH–DCs. At 48 h after nucleofection, supernatants were collected for TNF-α, IL-6, IL-1β and IL-12p70 ELISA analyses. The pshS1NH–DCs secreted significantly higher level of Th1 cytokines than the pNC-GFP-DCs and non-nucleofected DCs (P < 0.05). Data shown are the means ± SEM and representative of six independent experiments. The significant differences between pshS1NH–DCs and control were determined by ANOVA

    Techniques Used: Enzyme-linked Immunosorbent Assay, Control



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    specified nucleofection buffer  (Amaxa)
    90
    Amaxa specified nucleofection buffer
    Phenotypic analysis of DCs. At 24 h after <t>nucleofection,</t> DCs were harvested and stained with mAbs against DCs surface markers or appropriate isotype control antibody as described in “Materials and methods”. Percentage of positive DCs is indicated. One representative experiment of five is shown
    Specified Nucleofection Buffer, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/specified nucleofection buffer/product/Amaxa
    Average 90 stars, based on 1 article reviews
    specified nucleofection buffer - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Phenotypic analysis of DCs. At 24 h after nucleofection, DCs were harvested and stained with mAbs against DCs surface markers or appropriate isotype control antibody as described in “Materials and methods”. Percentage of positive DCs is indicated. One representative experiment of five is shown

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Enhanced activation of human dendritic cells by silencing SOCS1 and activating TLRs simultaneously

    doi: 10.1007/s00262-012-1218-4

    Figure Lengend Snippet: Phenotypic analysis of DCs. At 24 h after nucleofection, DCs were harvested and stained with mAbs against DCs surface markers or appropriate isotype control antibody as described in “Materials and methods”. Percentage of positive DCs is indicated. One representative experiment of five is shown

    Article Snippet: Nucleofection DCs were harvested at day 6 of culture, washed once in PBS/1%BSA and resuspended in specified nucleofection buffer from Amaxa (Germany) at a final concentration of 2 × 10 7 cells/ml.

    Techniques: Staining, Control

    Th1 cytokines secreted by pshS1NH–DCs were higher than those secreted by non-nucleofected DCs and pNC-GFP-DCs. 1 non-nucleofected DCs; 2 pNC-GFP-DCs; 3 pshS1NH–DCs. At 48 h after nucleofection, supernatants were collected for TNF-α, IL-6, IL-1β and IL-12p70 ELISA analyses. The pshS1NH–DCs secreted significantly higher level of Th1 cytokines than the pNC-GFP-DCs and non-nucleofected DCs (P < 0.05). Data shown are the means ± SEM and representative of six independent experiments. The significant differences between pshS1NH–DCs and control were determined by ANOVA

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Enhanced activation of human dendritic cells by silencing SOCS1 and activating TLRs simultaneously

    doi: 10.1007/s00262-012-1218-4

    Figure Lengend Snippet: Th1 cytokines secreted by pshS1NH–DCs were higher than those secreted by non-nucleofected DCs and pNC-GFP-DCs. 1 non-nucleofected DCs; 2 pNC-GFP-DCs; 3 pshS1NH–DCs. At 48 h after nucleofection, supernatants were collected for TNF-α, IL-6, IL-1β and IL-12p70 ELISA analyses. The pshS1NH–DCs secreted significantly higher level of Th1 cytokines than the pNC-GFP-DCs and non-nucleofected DCs (P < 0.05). Data shown are the means ± SEM and representative of six independent experiments. The significant differences between pshS1NH–DCs and control were determined by ANOVA

    Article Snippet: Nucleofection DCs were harvested at day 6 of culture, washed once in PBS/1%BSA and resuspended in specified nucleofection buffer from Amaxa (Germany) at a final concentration of 2 × 10 7 cells/ml.

    Techniques: Enzyme-linked Immunosorbent Assay, Control